1, Antifungal activity of juglone and its derivative 5,8-dihydroxy-1,4-naphthoquinone.

2, Fluorochrome stain assay certified that juglone can induce apoptosis of LS-174T cells.

3, In this paper, the effect of Juglone on LS-174T cell growth and the mechanism of effects were investigated.

4, Juglone product was obtained from bark of J. mandshurica by ultrasonic extraction and vacuum distillation, and the yield of product was 0.09 %.

5, The results suggest that the one-pot-method was an efficient and convenient synthesis approach of juglone.

6, The results showed that the optimum ultrasound extraction conditions for juglone were as follows:extraction time was 1.